Fast-fluorescence dynamics in nonratiometric calcium indicators.
- Gersbach M Ecole Polytechnique Fédérale de Lausanne, Quantum Architecture Group, Lausanne, Switzerland.
- Boiko DL
- Niclass C
- Petersen CC
- Charbon E
- 2009-02-03
Published in:
- Optics letters. - 2009
English
The fluorescence decay of high-affinity nonratiometric Ca2+ indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a model for accurate measurements of the free Ca2+ concentration and dissociation constants of nonratiometric calcium indicators.
- Language
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- English
- Open access status
- green
- Identifiers
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- DOI 10.1364/ol.34.000362
- PMID 19183659
- Persistent URL
- https://fredi.hepvs.ch/global/documents/96827
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