Mouse intestinal organoid time-course experiments from single cells

Serra, Denise; Mayr, Urs; Boni, Andrea; Lukonin, Ilya; Rempfler, Markus; Meylan, Ludivine Challet; Stadler, Michael B.; Strnad, Petr; Papasaikas, Panagiotis; Vischi, Dario; Waldt, Annick; Roma, Guglielmo; Liberali, Prisca

doi:10.21203/rs.2.9220/v1

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Journal article

Mouse intestinal organoid time-course experiments from single cells

Serra, Denise ORCID Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland
Mayr, Urs ORCID Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland
Boni, Andrea Viventis Microscopy Sàrl, EPFL Innovation Park, Lausanne, Switzerland
Lukonin, Ilya Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland
Rempfler, Markus Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland
Meylan, Ludivine Challet Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland
Stadler, Michael B. Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland
Strnad, Petr Viventis Microscopy Sàrl, EPFL Innovation Park, Lausanne, Switzerland
Papasaikas, Panagiotis Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland
Vischi, Dario Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland
Waldt, Annick Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland
Roma, Guglielmo Novartis Institutes for BioMedical Research, Novartis Pharma AG, Basel, Switzerland
Liberali, Prisca Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland

English Abstract
Organoids recapitulate the self-organizing capacity of stem cells and the tissue organization of the original organ in a controlled and trackable environment. Intestinal organoids, in particular, can develop from a single cell into a fully-grown structure that contains most of the cell types, patterns, and morphogenetic properties of the adult intestine. Here we present a protocol for high-throughput organoid culture in multi-well plate format combined with high content immunofluorescence imaging and RNA extraction. Our protocol allows recording and analysis of thousands of organoids during several days of development.

Language

English

Open access status

green

Identifiers

DOI 10.21203/rs.2.9220/v1

Persistent URL

https://fredi.hepvs.ch/global/documents/73634

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