Journal article
Live imaging of Mycobacterium marinum infection in Dictyostelium discoideum.
- Barisch C Département de Biochimie, Faculté des Sciences, Université de Genève, Sciences II, 30 Quai Ernest Ansermet, 1211, Genève-4, Switzerland.
- López-Jiménez AT
- Soldati T
- 2015-03-18
Published in:
- Methods in molecular biology (Clifton, N.J.). - 2015
English
The Dictyostelium discoideum-Mycobacterium marinum host-pathogen system is a recently established and powerful model system for mycobacterial infection. In this chapter, two simple protocols for live imaging of Dictyostelium discoideum infection are described. The first method is used to monitor the dynamics of recruitment of GFP-tagged Dictyostelium discoideum proteins at single time-points corresponding to the main stages of the infection (1.5-72 h post infection). The second method focuses at the early stages of the establishment of an infection (0-3 h post infection). In addition, several procedures to improve the imaging of the bacterium-containing compartment are described. Basic bacterial parameters such as bacterial growth and the recruitment of host proteins to the bacterium-containing compartment can be easily and precisely quantified using macros for ImageJ. These methods can be adapted to monitoring mycobacteria infection in other systems using mammalian cells.
- Language
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- English
- Open access status
- closed
- Identifiers
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- DOI 10.1007/978-1-4939-2450-9_23
- PMID 25779329
- Persistent URL
- https://fredi.hepvs.ch/global/documents/50259
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