Characterization of the cleavage site and the recognition sequence of the I-CreI DNA endonuclease encoded by the chloroplast ribosomal intron of Chlamydomonas reinhardtii.
- Dürrenberger F Department of Molecular Biology, University of Geneva, Switzerland.
- Rochaix JD
- 1993-01-01
Published in:
- Molecular & general genetics : MGG. - 1993
Animals
Base Sequence
Chlamydomonas reinhardtii
Chloroplasts
DNA Restriction Enzymes
Deoxyribonucleases, Type II Site-Specific
Escherichia coli
Genetic Variation
Introns
Molecular Sequence Data
RNA, Ribosomal, 23S
Recombinant Proteins
Substrate Specificity
English
The chloroplast ribosomal intron of Chlamydomonas reinhardtii encodes a sequence-specific DNA endonuclease (I-CreI), which is most probably involved in the mobility of this intron. Here we show that I-CreI generates a 4 bp staggered cleavage just downstream of the intron insertion site. The I-CreI recognition sequence is 19-24 bp in size and is located asymmetrically around the intron insertion site. Screening of natural variants of the I-CreI recognition sequence indicates that the I-CreI endonuclease tolerates single and even multiple base changes within its recognition sequence.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1007/bf00277141
- PMID 8437585
- Persistent URL
- https://fredi.hepvs.ch/global/documents/39049
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