Journal article
Flavonol-mediated stabilization of PIN efflux complexes regulates polar auxin transport.
- Teale WD Institute of Biology II, University of Freiburg, Freiburg, Germany.
- Pasternak T Institute of Biology II, University of Freiburg, Freiburg, Germany.
- Dal Bosco C Institute of Biology II, University of Freiburg, Freiburg, Germany.
- Dovzhenko A Institute of Biology II, University of Freiburg, Freiburg, Germany.
- Kratzat K Institute of Biology II, University of Freiburg, Freiburg, Germany.
- Bildl W Institute of Physiology II, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
- Schwörer M Institute of Biology II, University of Freiburg, Freiburg, Germany.
- Falk T Institute for Computer Science, University of Freiburg, Freiburg, Germany.
- Ruperti B Department of Agronomy, Food, Natural resources, Animals and Environment-DAFNAE, University of Padova, Padova, Italy.
- Schaefer JV High-Throughput Binder Selection Facility, Department of Biochemistry, University of Zurich, Zurich, Switzerland.
- Shahriari M Institute of Biology II, University of Freiburg, Freiburg, Germany.
- Pilgermayer L Institute of Biology II, University of Freiburg, Freiburg, Germany.
- Li X Sino German Joint Research Center for Agricultural Biology, and State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, China.
- Lübben F Institute of Biology II, University of Freiburg, Freiburg, Germany.
- Plückthun A High-Throughput Binder Selection Facility, Department of Biochemistry, University of Zurich, Zurich, Switzerland.
- Schulte U Institute of Physiology II, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
- Palme K Institute of Biology II, University of Freiburg, Freiburg, Germany.
- 2020-11-13
Published in:
- The EMBO journal. - 2020
English
The transport of auxin controls the rate, direction and localization of plant growth and development. The course of auxin transport is defined by the polar subcellular localization of the PIN proteins, a family of auxin efflux transporters. However, little is known about the composition and regulation of the PIN protein complex. Here, using blue-native PAGE and quantitative mass spectrometry, we identify native PIN core transport units as homo- and heteromers assembled from PIN1, PIN2, PIN3, PIN4 and PIN7 subunits only. Furthermore, we show that endogenous flavonols stabilize PIN dimers to regulate auxin efflux in the same way as does the auxin transport inhibitor 1-naphthylphthalamic acid (NPA). This inhibitory mechanism is counteracted both by the natural auxin indole-3-acetic acid and by phosphomimetic amino acids introduced into the PIN1 cytoplasmic domain. Our results lend mechanistic insights into an endogenous control mechanism which regulates PIN function and opens the way for a deeper understanding of the protein environment and regulation of the polar auxin transport complex.
- Language
-
- English
- Open access status
- hybrid
- Identifiers
-
- DOI 10.15252/embj.2020104416
- PMID 33185277
- Persistent URL
- https://fredi.hepvs.ch/global/documents/284188
Statistics
Document views: 45
File downloads: