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CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors.
Journal article

CHO and HEK293 Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors.

  • Hacker DL Protein Production and Structure Core Facility (PPSCF), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. david.hacker@epfl.ch.
  • Durrer L Protein Production and Structure Core Facility (PPSCF), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
  • Quinche S Protein Production and Structure Core Facility (PPSCF), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
  • 2018-09-23
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2018
CHO cells
Disposable bioreactors
HEK293 cells
Suspension cells
Transfection
Animals
Bioreactors
CHO Cells
Cricetinae
Cricetulus
HEK293 Cells
Humans
Recombinant Proteins
Transfection
English Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells are the two most popular mammalian hosts for the production of recombinant proteins. In this chapter the suspension cultivation and transfection of these cells in small-scale disposable bioreactors is described. The TubeSpin bioreactor 50 and TubeSpin bioreactor 600 are designed for the cultivation of suspension cells by orbital shaking and have maximum working volumes of about 15 mL and 400 mL, respectively.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://fredi.hepvs.ch/global/documents/204589
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