Matrix-assisted laser desorption ionization-time of flight mass spectrometry for the identification of clinically relevant bacteria.
- 2011-02-02
Published in:
- PloS one. - 2011
Bacteria
Enterococcus
Gardnerella
RNA, Ribosomal, 16S
Sequence Analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Staphylococcus
Streptococcus
English
BACKGROUND
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) allows rapid and reliable identification of microorganisms, particularly clinically important pathogens.
METHODOLOGY/PRINCIPAL FINDINGS
We compared the identification efficiency of MALDI-TOF MS with that of Phoenix®, API® and 16S ribosomal DNA sequence analysis on 1,019 strains obtained from routine diagnostics. Further, we determined the agreement of MALDI-TOF MS identifications as compared to 16S gene sequencing for additional 545 strains belonging to species of Enterococcus, Gardnerella, Staphylococcus, and Streptococcus. For 94.7% of the isolates MALDI-TOF MS results were identical with those obtained with conventional systems. 16S sequencing confirmed MALDI-TOF MS identification in 63% of the discordant results. Agreement of identification of Gardnerella, Enterococcus, Streptococcus and Staphylococcus species between MALDI-TOF MS and traditional method was high (Crohn's kappa values: 0.9 to 0.93).
CONCLUSIONS/SIGNIFICANCE
MALDI-TOF MS represents a rapid, reliable and cost-effective identification technique for clinically relevant bacteria.
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) allows rapid and reliable identification of microorganisms, particularly clinically important pathogens.
METHODOLOGY/PRINCIPAL FINDINGS
We compared the identification efficiency of MALDI-TOF MS with that of Phoenix®, API® and 16S ribosomal DNA sequence analysis on 1,019 strains obtained from routine diagnostics. Further, we determined the agreement of MALDI-TOF MS identifications as compared to 16S gene sequencing for additional 545 strains belonging to species of Enterococcus, Gardnerella, Staphylococcus, and Streptococcus. For 94.7% of the isolates MALDI-TOF MS results were identical with those obtained with conventional systems. 16S sequencing confirmed MALDI-TOF MS identification in 63% of the discordant results. Agreement of identification of Gardnerella, Enterococcus, Streptococcus and Staphylococcus species between MALDI-TOF MS and traditional method was high (Crohn's kappa values: 0.9 to 0.93).
CONCLUSIONS/SIGNIFICANCE
MALDI-TOF MS represents a rapid, reliable and cost-effective identification technique for clinically relevant bacteria.
- Language
-
- English
- Open access status
- gold
- Identifiers
-
- DOI 10.1371/journal.pone.0016424
- PMID 21283545
- Persistent URL
- https://fredi.hepvs.ch/global/documents/189244
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