Addition of G418 and other aminoglycoside antibiotics to mammalian cells results in the release of GPI-anchored proteins.
- Küng M Institute of Biochemistry and Molecular Biology, University of Bern, Switzerland.
- Stadelmann B
- Brodbeck U
- Bütikofer P
- 1997-06-16
Published in:
- FEBS letters. - 1997
Alkaline Phosphatase
Animals
Anti-Bacterial Agents
COS Cells
Chlorocebus aethiops
Dose-Response Relationship, Drug
Enzyme Activation
Erythrocytes
Gentamicins
Glycosylphosphatidylinositols
Humans
Membrane Proteins
Time Factors
Transfection
English
Resistance to the neomycin analogue G418 forms the basis of a dominant marker selection system for mammalian cells transfected with the bacterial neomycin gene. We found that COS-1 cells stably transfected with the neomycin resistance gene had a greater than 50% reduction in cell-associated glycosylphosphatidylinositol (GPI)-anchored alkaline phosphatase (AP). A similarly reduced amount of AP was also observed in wild-type COS-1 cells incubated in the presence of G418 or other aminoglycoside antibiotics. The AP was released from cells into the culture supernatant in its GPI-anchored form. Our data suggest that the G418-induced reduction of AP involves a vesiculation process of COS-1 cells.
- Language
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- English
- Open access status
- bronze
- Identifiers
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- DOI 10.1016/s0014-5793(97)00452-3
- PMID 9224684
- Persistent URL
- https://fredi.hepvs.ch/global/documents/157621
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