Journal article
Enzyme assays for high-throughput screening.
- Goddard JP Department of Chemistry & Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland.
- Reymond JL
- 2004-09-11
Published in:
- Current opinion in biotechnology. - 2004
Catalysis
Enzyme-Linked Immunosorbent Assay
Enzymes
Fluorescence Resonance Energy Transfer
Gene Expression Profiling
Mass Spectrometry
Oligonucleotide Array Sequence Analysis
Peptide Mapping
Spectrometry, Fluorescence
English
Assaying enzyme-catalyzed transformations in high-throughput is crucial to enzyme discovery, enzyme engineering and the drug discovery process. In enzyme assays, catalytic activity is detected using labelled substrates or indirect sensor systems that produce a detectable spectroscopic signal upon reaction. Recent advances in the development of high-throughput enzyme assays have identified new labels and chromophores to detect a wide range of enzymes activities. Enzyme activity profiling and fingerprinting have also been used as tools for identification and classification, while microarray formats have been devised to increase throughput.
- Language
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- English
- Open access status
- closed
- Identifiers
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- DOI 10.1016/j.copbio.2004.06.008
- PMID 15358001
- Persistent URL
- https://fredi.hepvs.ch/global/documents/143815
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